DATASETS FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL

We found 6 data for "FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL"

LIST DATASET

NO. DATASET TITLE
1.
ASSOCIATION OF SOIL FUNGAL COMMUNITY COMPOSITION WITH INCIDENCE OF FUSARIUM WILT OF BANANA IN MALAYSIA
DESCRIPTION : Banana (Musa spp.), an important food crop in many parts of the world, is threatened by a deadly wilt disease caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4). Increasing evidence indicates that plant actively recruits beneficial microbes in the rhizosphere to suppress soil-borne pathogens. Hence, studies on the composition and diversity of the root-associated microbial communities are important for banana health. Research on beneficial microbial communities has focused on bacteria, although fungi can also influence soil-borne disease. Here, high-throughput sequencing targeting the fungal internal transcribed spacer (ITS) was employed to systematically characterize the difference in the soil fungal community associated with Fusarium wilt (FW) of banana. The community structure of fungi in the healthy and TR4-infected rhizospheres was significantly different compared with that of bulk soil within the same farm. The rhizosphere soils of infected plants exhibited higher richness and diversity compared with healthy plants, with significant abundance of Fusarium genus at 14%. In the healthy rhizosphere soil, Penicillium spp. were more abundant at 7% and positively correlated with magnesium. This study produced a detailed description of fungal community structure in healthy and TR4-infected banana soils in Malaysia and identified candidate biomarker taxa that may be associated with FW disease promotion and suppression. The findings also expand the global inventory of fungal communities associated with the components of asymptomatic and symptomatic banana plants infected by TR4.
CONTRIBUTOR : NOORADIBAH BINTI ISMAIL (11/05/2023 19:35 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 4711 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 6 ;
KEYWORD : [internal transcribed spacer] , [ rhizosphere] , [ richness] , [ soil microbiome] , [ soil physicochemical properties] , [ TR4]
2.
DRAFT GENOME SEQUENCE OF MYO-INOSITOL UTILIZING AEROMONAS DHAKENSIS 1P11S3 ISOLATED FROM STRIPED CATFISH (PANGASIANODON HYPOPTHALMUS) IN A LOCAL FISH FARM IN MALAYSIA
DESCRIPTION : A hypervirulent pathotype of A. hydrophila (vAh) is responsible for Motile Aeromonas Septicemia (MAS) and causes mass mortalities among farmed carp and catfish species in the USA and China. One unique phenotype for vAh among other A. hydrophila strains is the ability to utilize myo-inositol as a sole carbon source. While screening for Aeromonas isolates from diseased fish that can grow using myo-inositol as a sole carbon source, A. dhakensis 1P11S3 was isolated from the spleen of striped catfish (Pangasianodon hypopthalmus) displaying clinical MAS symptoms from a freshwater farm in Malaysia. Aeromonas dhakensis is also an important pathogen in aquaculture, and in this study, we report the draft genome sequence for A. dhakensis 1P11S3, that utilize myo-inositol as a sole carbon source.
CONTRIBUTOR : MOHD TERMIZI BIN YUSOF (11/05/2023 17:00 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2707 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 0 ; DOWNLOAD : 0 ;
KEYWORD : [Draft genome] , [ myo-inositol A.] , [ dhakensis] , [ Malaysia]
3.
RNA-SEQUENCING OF LYMPH, SPLEEN, AND THYMUS TRANSCRIPTOME OF PENINSULAR MALAYSIA M. FASCICULARIS
DESCRIPTION : The cynomolgus macaque (Macaca fascicularis) is an extensively utilised nonhuman primate model for biomedical research due to its biological, behavioural, and genetic similarities to humans. Genomic information of cynomolgus macaque is vital for research in various fields; however, there is presently a shortage of genomic information on the Malaysian cynomolgus macaque. This study aimed to sequence, assemble, annotate, and profile the Peninsular Malaysian cynomolgus macaque transcriptome derived from three tissues (lymph node, spleen, and thymus) using RNA sequencing (RNA-Seq) technology. A total of 174,208,078 paired end 70 base pair sequencing reads were obtained from the Illumina Hi-Seq 2500 sequencer. The overall mapping percentage of the sequencing reads to the M. fascicularis reference genome ranged from 53-63%. Categorisation of expressed genes to Gene Ontology (GO) and KEGG pathway categories revealed that GO terms with the highest number of associated expressed genes include Cellular process, Catalytic activity, and Cell part, while for pathway categorisation, the majority of expressed genes in lymph node, spleen, and thymus fall under the Global overview and maps pathway category, while 266, 221, and 138 genes from lymph node, spleen, and thymus were respectively enriched in the Immune system category. Enriched Immune system pathways include Platelet activation pathway, Antigen processing and presentation, B cell receptor signalling pathway, and Intestinal immune network for IgA production. Differential gene expression analysis among the three tissues revealed 574 differentially expressed genes (DEG) between lymph and spleen, 5402 DEGs between lymph and thymus, and 7008 DEGs between spleen and thymus. Venn diagram analysis of expressed genes revealed a total of 2,630, 253, and 279 tissue-specific genes respectively for lymph node, spleen, and thymus tissues. This is the first time the lymph node, spleen, and thymus transcriptome of the Peninsular Malaysian cynomolgus macaque have been sequenced via RNA-Seq. Novel transcriptomic data will further enrich the present M. fascicularis genomic database and provide future research potentials, including novel transcript discovery, comparative studies, and molecular markers development.
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 16:25 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2702 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Genomic database; Lymph node; Macaca fascicularis; Peninsular malaysian; RNA sequencing; Spleen; Thymus; Transcriptome]
4.
FLORAL MICROMORPHOLOGY AND TRANSCRIPTOME ANALYSES OF A FRAGRANT VANDACEOUS ORCHID, VANDA MIMI PALMER, FOR ITS FRAGRANCE PRODUCTION SITES
DESCRIPTION : Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the flowers. Results Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifically linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at different time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. Conclusions Knowledge of the VMP floral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and flower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the flower morphology to maximize the reproduction success of this plant.
CONTRIBUTOR : PARAMESWARI A/P NAMASIVAYAM (11/05/2023 16:16 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2380 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Fragrance] , [ Monoterpene synthase] , [ Morphology] , [ Orchid] , [ Stomata] , [ Trichome]
5.
GENE EXPRESSION ANALYSIS REVEALS THE CONCURRENT ACTIVATION OF PRO-APOPTOTIC AND ANTIOXIDANT-DEFENSIVE MECHANISMS IN FLAVOKAWAIN B AND FIH-TREATED CERVICAL CANCER HELA CELLS
DESCRIPTION : To examine the difference in gene expression of HeLa cells after 48 hours of treatment with 7 µg/mL FKB and 15 µg/mL FIH after 48 hours. 3 sets of sample were prepared; the untreated HeLa cells, 7 µg/mL FKB-treated HeLa cells after 48 hours, 15 µg/mL of FIH-treated HeLa cells after 48 hours
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 14:58 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2566 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [HeLa cells] , [ cervical cancer]
6.
REGULATED GENE EXPRESSION FOR BREAST CANCER CELLS TREATED WITH NLC-CITRAL FOR 48 HOURS
DESCRIPTION : To further develop our gene expression study, we have employed the microrarray expression to study the regulate gene expression on breast cancer cells after 48 hours of treatments
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 14:48 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2444 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [microrarray] , [ breast cancer] , [ NLC-Citral]

Organization (Faculty)

Fakulti Perubatan Dan Sains Kesihatan [25]
Fakulti Sains [18]
Fakulti Bioteknologi Dan Sains Biomolekul [6]
Fakulti Sains Komputer Dan Teknologi Maklumat [1]
Fakulti Bahasa Moden Dan Komunikasi [2]
Fakulti Kejuruteraan [2]
Institut Pertanian Tropika Dan Sekuriti Makanan [1]
Institut Gerontologi [2]
Institut Biosains [2]
Fakulti Sains Dan Teknologi Makanan [1]
Pusat Pembangunan Maklumat Dan Komunikasi [1]
Fakulti Ekonomi Dan Pengurusan [2]
Fakulti Perubatan Veterinar [4]
Fakulti Pertanian [8]
Fakulti Ekologi Manusia [2]
Institut Penyelidikan Produk Halal [1]
Institut Penyelidikan Matematik [1]
Fakulti Sains Pertanian Dan Makanan [2]
Institut Perhutanan Tropika Dan Produk Hutan [2]
Kampus Upm Bintulu [1]
Fakulti Perhutanan [6]

Filter By

Subject Area

Agricultural And Biological Sciences [13]
Biochemistry, Genetics And Molecular Biology [12]
Chemical Engineering [1]
Chemistry [11]
Computer Science [3]
Earth And Planetary Sciences [1]
Economics, Econometrics And Finance [1]
Energy [4]
Engineering [3]
Environmental Science [16]
Health Professions [1]
Immunology And Microbiology [4]
Materials Science [14]
Mathematics [1]
Medicine [29]
Pharmacology, Toxicology And Pharmaceutics [2]
Psychology [1]
Social Sciences [7]
Veterinary [5]