#RDRepo#

NO.	DATASET TITLE
1.	RNA-SEQUENCING OF LYMPH, SPLEEN, AND THYMUS TRANSCRIPTOME OF PENINSULAR MALAYSIA M. FASCICULARIS DESCRIPTION : The cynomolgus macaque (Macaca fascicularis) is an extensively utilised nonhuman primate model for biomedical research due to its biological, behavioural, and genetic similarities to humans. Genomic information of cynomolgus macaque is vital for research in various fields; however, there is presently a shortage of genomic information on the Malaysian cynomolgus macaque. This study aimed to sequence, assemble, annotate, and profile the Peninsular Malaysian cynomolgus macaque transcriptome derived from three tissues (lymph node, spleen, and thymus) using RNA sequencing (RNA-Seq) technology. A total of 174,208,078 paired end 70 base pair sequencing reads were obtained from the Illumina Hi-Seq 2500 sequencer. The overall mapping percentage of the sequencing reads to the M. fascicularis reference genome ranged from 53-63%. Categorisation of expressed genes to Gene Ontology (GO) and KEGG pathway categories revealed that GO terms with the highest number of associated expressed genes include Cellular process, Catalytic activity, and Cell part, while for pathway categorisation, the majority of expressed genes in lymph node, spleen, and thymus fall under the Global overview and maps pathway category, while 266, 221, and 138 genes from lymph node, spleen, and thymus were respectively enriched in the Immune system category. Enriched Immune system pathways include Platelet activation pathway, Antigen processing and presentation, B cell receptor signalling pathway, and Intestinal immune network for IgA production. Differential gene expression analysis among the three tissues revealed 574 differentially expressed genes (DEG) between lymph and spleen, 5402 DEGs between lymph and thymus, and 7008 DEGs between spleen and thymus. Venn diagram analysis of expressed genes revealed a total of 2,630, 253, and 279 tissue-specific genes respectively for lymph node, spleen, and thymus tissues. This is the first time the lymph node, spleen, and thymus transcriptome of the Peninsular Malaysian cynomolgus macaque have been sequenced via RNA-Seq. Novel transcriptomic data will further enrich the present M. fascicularis genomic database and provide future research potentials, including novel transcript discovery, comparative studies, and molecular markers development. CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 16:25 PM) FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 2702 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Genomic database; Lymph node; Macaca fascicularis; Peninsular malaysian; RNA sequencing; Spleen; Thymus; Transcriptome]
2.	THE MALAYSIAN GESTATIONAL DIABETES AND PREVENTION OF DIABTES STUDY (MY GODDESS) DESCRIPTION : Over 50% of women with a history of gestational diabetes mellitus (GDM) will develop type 2 diabetes (T2D) in later life. Asian women experience a disproportionate risk of both GDM and T2D compared to women from other ethnic backgrounds. Lifestyle interventions and behavior change can delay or even prevent the onset of T2D. We have developed a digitalized diabetes prevention intervention for the prevention of T2D in Malaysian women with GDM. CONTRIBUTOR : CHEW BOON HOW (11/05/2023 16:18 PM) FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN PUBLICATION : 2 ; FILE IN : HAS VIEWS : 1305 ; FOLDER IN PROJECT : 0 ; FILE IN PROJECT : 0 ; DOWNLOAD : 0 ; KEYWORD : [gestational diabetes mellitus] , [ diabetes prevention] , [ randomized controlled trial] , [ mobile app]
3.	HUMAN MESENCHYMAL STEM CELLS-MEDIATED TRANSCRIPTOMIC REGULATION OF LEUKEMIC CELLS IN DELIVERING ANTI-TUMORIGENIC EFFECTS DESCRIPTION : Treatment of leukemia has become much difficult because of resistance to the existing anticancer therapies. This has thus expedited the search for alternativ therapies, and one of these is the exploitation of mesenchymal stem cells (MSCs) towards control of tumor cells. The present study investigated the effect of human umbilical cord-derived MSCs (UC-MSCs) on the proliferation of leukemic cells and gauged the transcriptomic modulation and the signaling pathways potentially affected by UC-MSCs. The inhibition of growth of leukemic tumor cell lines was assessed by proliferation assays, apoptosis and cell cycle analysis. BV173 and HL-60 cells were further analyzed using microarray gene expression profiling. The microarray results were validated by RT-qPCR and western blot assay for the corresponding expression of genes and proteins. The UC-MSCs attenuated leukemic cell viability and proliferation in a dose-dependent manner without inducing apoptosis. Cell cycle analysis revealed that the growth of tumor cells was arrested at the G0/G1 phase. The microarray results identified that HL-60 and BV173 share 35 differentially expressed genes (DEGs) (same expression direction) in the presence of UC-MSCs. In silico analysis of these selected DEGs indicated a significant influence in the cell cycle and cell cycle-related biological processes and signaling pathways. Among these, the expression of DBF4, MDM2, CCNE2, CDK6, CDKN1A, and CDKN2A was implicated in six different signaling pathways that play a pivotal role in the anti-tumorigenic activity exerted by UC-MSCs. The UC-MSCs perturbate the cell cycle process of leukemic cells via dysregulation of tumor suppressor and oncogene expression. CONTRIBUTOR : LING KING HWA (11/05/2023 14:43 PM) FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 557 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [cell cycle; gene expression profiling; leukemia; mesenchymal stem cells]
4.	ATR-FTIR IDENTIFICATION USING SPECTRAL LIBRARY DATABASES DESCRIPTION : With the help of the three IR spectral libraries of General Library IR (350 spectra), SWGDRUG IR library-31 May 2018 (503 spectra) and Demo Library (200 spectra), all experimental FTIR spectra were successfully identified after spectral library search. The spectral matching operation was performed by software algorithm (standard search algorithm) with one main component mode in OPUS (Bruker). CONTRIBUTOR : MOHD RASHIDI BIN ABDULL MANAP (11/05/2023 10:10 AM) FACULTY : FAKULTI SAINS PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 1192 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 5 ; KEYWORD : [Fourier Transform Infrared Spectroscopy] , [ Spectral Analysis] , [ Database]

1.

RNA-SEQUENCING OF LYMPH, SPLEEN, AND THYMUS TRANSCRIPTOME OF PENINSULAR MALAYSIA M. FASCICULARIS
DESCRIPTION : The cynomolgus macaque (Macaca fascicularis) is an extensively utilised nonhuman primate model for biomedical research due to its biological, behavioural, and genetic similarities to humans. Genomic information of cynomolgus macaque is vital for research in various fields; however, there is presently a shortage of genomic information on the Malaysian cynomolgus macaque. This study aimed to sequence, assemble, annotate, and profile the Peninsular Malaysian cynomolgus macaque transcriptome derived from three tissues (lymph node, spleen, and thymus) using RNA sequencing (RNA-Seq) technology. A total of 174,208,078 paired end 70 base pair sequencing reads were obtained from the Illumina Hi-Seq 2500 sequencer. The overall mapping percentage of the sequencing reads to the M. fascicularis reference genome ranged from 53-63%. Categorisation of expressed genes to Gene Ontology (GO) and KEGG pathway categories revealed that GO terms with the highest number of associated expressed genes include Cellular process, Catalytic activity, and Cell part, while for pathway categorisation, the majority of expressed genes in lymph node, spleen, and thymus fall under the Global overview and maps pathway category, while 266, 221, and 138 genes from lymph node, spleen, and thymus were respectively enriched in the Immune system category. Enriched Immune system pathways include Platelet activation pathway, Antigen processing and presentation, B cell receptor signalling pathway, and Intestinal immune network for IgA production. Differential gene expression analysis among the three tissues revealed 574 differentially expressed genes (DEG) between lymph and spleen, 5402 DEGs between lymph and thymus, and 7008 DEGs between spleen and thymus. Venn diagram analysis of expressed genes revealed a total of 2,630, 253, and 279 tissue-specific genes respectively for lymph node, spleen, and thymus tissues. This is the first time the lymph node, spleen, and thymus transcriptome of the Peninsular Malaysian cynomolgus macaque have been sequenced via RNA-Seq. Novel transcriptomic data will further enrich the present M. fascicularis genomic database and provide future research potentials, including novel transcript discovery, comparative studies, and molecular markers development.
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 16:25 PM)

FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 2702 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Genomic database; Lymph node; Macaca fascicularis; Peninsular malaysian; RNA sequencing; Spleen; Thymus; Transcriptome]

2.

THE MALAYSIAN GESTATIONAL DIABETES AND PREVENTION OF DIABTES STUDY (MY GODDESS)
DESCRIPTION : Over 50% of women with a history of gestational diabetes mellitus (GDM) will develop type 2 diabetes (T2D) in later life. Asian women experience a disproportionate risk of both GDM and T2D compared to women from other ethnic backgrounds. Lifestyle interventions and behavior change can delay or even prevent the onset of T2D. We have developed a digitalized diabetes prevention intervention for the prevention of T2D in Malaysian women with GDM.
CONTRIBUTOR : CHEW BOON HOW (11/05/2023 16:18 PM)

FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 2 ; FILE IN :
HAS VIEWS : 1305 ; FOLDER IN PROJECT : 0 ; FILE IN PROJECT : 0 ; DOWNLOAD : 0 ;
KEYWORD : [gestational diabetes mellitus] , [ diabetes prevention] , [ randomized controlled trial] , [ mobile app]

3.

HUMAN MESENCHYMAL STEM CELLS-MEDIATED TRANSCRIPTOMIC REGULATION OF LEUKEMIC CELLS IN DELIVERING ANTI-TUMORIGENIC EFFECTS
DESCRIPTION : Treatment of leukemia has become much difficult because of resistance to the existing anticancer therapies. This has thus expedited the search for alternativ therapies, and one of these is the exploitation of mesenchymal stem cells (MSCs) towards control of tumor cells. The present study investigated the effect of human umbilical cord-derived MSCs (UC-MSCs) on the proliferation of leukemic cells and gauged the transcriptomic modulation and the signaling pathways potentially affected by UC-MSCs. The inhibition of growth of leukemic tumor cell lines was assessed by proliferation assays, apoptosis and cell cycle analysis. BV173 and HL-60 cells were further analyzed using microarray gene expression profiling. The microarray results were validated by RT-qPCR and western blot assay for the corresponding expression of genes and proteins. The UC-MSCs attenuated leukemic cell viability and proliferation in a dose-dependent manner without inducing apoptosis. Cell cycle analysis revealed that the growth of tumor cells was arrested at the G0/G1 phase. The microarray results identified that HL-60 and BV173 share 35 differentially expressed genes (DEGs) (same expression direction) in the presence of UC-MSCs. In silico analysis of these selected DEGs indicated a significant influence in the cell cycle and cell cycle-related biological processes and signaling pathways. Among these, the expression of DBF4, MDM2, CCNE2, CDK6, CDKN1A, and CDKN2A was implicated in six different signaling pathways that play a pivotal role in the anti-tumorigenic activity exerted by UC-MSCs. The UC-MSCs perturbate the cell cycle process of leukemic cells via dysregulation of tumor suppressor and oncogene expression.
CONTRIBUTOR : LING KING HWA (11/05/2023 14:43 PM)

FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 557 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [cell cycle; gene expression profiling; leukemia; mesenchymal stem cells]

4.

ATR-FTIR IDENTIFICATION USING SPECTRAL LIBRARY DATABASES
DESCRIPTION : With the help of the three IR spectral libraries of General Library IR (350 spectra), SWGDRUG IR library-31 May 2018 (503 spectra) and Demo Library (200 spectra), all experimental FTIR spectra were successfully identified after spectral library search. The spectral matching operation was performed by software algorithm (standard search algorithm) with one main component mode in OPUS (Bruker).
CONTRIBUTOR : MOHD RASHIDI BIN ABDULL MANAP (11/05/2023 10:10 AM)

FACULTY : FAKULTI SAINS
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 1192 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 5 ;
KEYWORD : [Fourier Transform Infrared Spectroscopy] , [ Spectral Analysis] , [ Database]

Fakulti Perubatan Dan Sains Kesihatan	[25]
Fakulti Sains	[18]
Fakulti Bioteknologi Dan Sains Biomolekul	[6]
Fakulti Sains Komputer Dan Teknologi Maklumat	[1]
Fakulti Bahasa Moden Dan Komunikasi	[2]
Fakulti Kejuruteraan	[2]
Institut Pertanian Tropika Dan Sekuriti Makanan	[1]
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Fakulti Sains Dan Teknologi Makanan	[1]
Pusat Pembangunan Maklumat Dan Komunikasi	[1]
Fakulti Ekonomi Dan Pengurusan	[2]
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Fakulti Sains Pertanian Dan Makanan	[2]
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