DATASETS BIOCHEMISTRY, GENETICS AND MOLECULAR BIOLOGY

We found 12 data for "BIOCHEMISTRY, GENETICS AND MOLECULAR BIOLOGY"

LIST DATASET

NO. DATASET TITLE
1.
CHANGES IN ANTHROPOMETRIC, BODY COMPOSITION, CLINICAL PARAMETERS, BIOCHEMICAL PROFILES, AND BIOMARKERS CONCENTRATION OF SUBCLINICAL ATHEROSCLEROSIS OF THE EBN INTERVENTION AMONG PREMENOPAUSAL WOMEN: A RANDOMIZED CONTROL TRIAL
DESCRIPTION : Introduction: The menopausal transition is an important physiological stage in the lives of women that is associated with the risk of cardiovascular disease later in life. Objective: This study aimed to describe changes in anthropometric measurements, body composition, clinical parameters, biochemical profiles, biomarker concentration, and carotid ultrasound to address risk factors for subclinical atherosclerosis disease among premenopausal women with borderline hypercholesterolemia before and after intervention. This was a 12-week randomized control trial comparing an intervention with an edible bird nest with a control. Subjects: The study involved 39 premenopausal women 40-55 years of age who, following inclusion and exclusion criteria, were allocated into two groups, an intervention group consuming one (1) pouch (250 g) of edible bird nest mix (EBN) daily for 12 weeks (between days 84 and 90 days from day 1 of menses) and the control group consuming the same weight, texture and taste of the placebo. Main outcome measures: Assessment before and after inclusion of the anthropometric status parameters [weight, height, body mass index, hip, waist to hip ratio (WHR), body composition (body fat, body age, whole body skeletal, trunk skeletal, arm skeletal, leg skeletal, total body subcutaneous, trunk subcutaneous, arm subcutaneous, leg subcutaneous, visceral fat), clinical parameters (blood pressure (systolic and diastolic), thickness of the carotid intima, biochemical profiles (lipid profile, lasting blood glucose), biomarker concentrations (APO AI, APO AII, APO B, APO CII, APO CIII, APO E). This study showed that the EBN intervention for premenopausal women with mild hypercholesterolemia resulted in slightly beneficial changes in anthropometric measurement, body composition, clinical parameters, and biomarker concentration associated with cardiovascular risk in older women. These findings present a potential for the use of EBN in individuals with borderline hypercholesterolemia to provide cholesterol lowering benefit based on an animal-based diet strategy that can improve overall health outcomes when reaching menopausal age.
CONTRIBUTOR : SALWANA BINTI AHMAD (25/12/2023 16:43 PM)
FACULTY : INSTITUT BIOSAINS
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 17 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 1 ;
KEYWORD : [Biochemical profile] , [ subclinical atherosclerosis] , [ carotid intima media thickness] , [ ovary size] , [ vascular aging] , [ premenopausal women.]
2.
EFFECT OF DIFFERENT DRYING TREATMENTS AND SOLVENT RATIOS ON PHYTOCHEMICAL CONSTITUENTS OF IPOMOEA AQUATICA AND CORRELATION WITH α-GLUCOSIDASE INHIBITORY ACTIVITY
DESCRIPTION : Ipomoea aquatica is an aquatic plant that is widely consumed in Southeast Asia as a vegetable. In this study, the influence of various ethanol ratios (0, 20, 50, 80, and 100%) as an extraction solvent and different drying methods including air drying (AD), sun drying (SD) and oven drying (OD) on phytochemical constituents of I. aquatica was investigated using a proton nuclear magnetic resonance (1H NMR) based metabolomics approach. The effect on α-glucosidase inhibitory activity and total phenolic content (TPC) was also examined. Clear discrimination was observed between different ethanol ratios and different drying processes by principal component analysis (PCA). The highest α-glucosidase inhibitory activity was observed for absolute ethanol extract from the OD method with IC50 value of 204.0 ± 59.0 µg/mL and TPC value of 22.0 ± 0.7 µg GAE/mg extract. Correlation between the α-glucosidase inhibitory activity and the metabolite were analyzed using a partial least square (PLS) analysis. The metabolites that are responsible for the activity were quercetin derivatives, chlorogenic acid derivatives, sucrose and fructose. This study highlights the basis for future investigations of I. aquatica as a source of food that has the potential for nutraceutical enhancement and as ingredient in medicinal preparation.
CONTRIBUTOR : AMIN BIN ISMAIL (12/05/2023 16:31 PM)
FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 3674 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 6 ;
KEYWORD : [1H NMR metabolomics] , [ Ipomoea aquatica] , [ Alpha-glucosidase inhibitory] , [ PLS]
3.
ASSOCIATION OF SOIL FUNGAL COMMUNITY COMPOSITION WITH INCIDENCE OF FUSARIUM WILT OF BANANA IN MALAYSIA
DESCRIPTION : Banana (Musa spp.), an important food crop in many parts of the world, is threatened by a deadly wilt disease caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4). Increasing evidence indicates that plant actively recruits beneficial microbes in the rhizosphere to suppress soil-borne pathogens. Hence, studies on the composition and diversity of the root-associated microbial communities are important for banana health. Research on beneficial microbial communities has focused on bacteria, although fungi can also influence soil-borne disease. Here, high-throughput sequencing targeting the fungal internal transcribed spacer (ITS) was employed to systematically characterize the difference in the soil fungal community associated with Fusarium wilt (FW) of banana. The community structure of fungi in the healthy and TR4-infected rhizospheres was significantly different compared with that of bulk soil within the same farm. The rhizosphere soils of infected plants exhibited higher richness and diversity compared with healthy plants, with significant abundance of Fusarium genus at 14%. In the healthy rhizosphere soil, Penicillium spp. were more abundant at 7% and positively correlated with magnesium. This study produced a detailed description of fungal community structure in healthy and TR4-infected banana soils in Malaysia and identified candidate biomarker taxa that may be associated with FW disease promotion and suppression. The findings also expand the global inventory of fungal communities associated with the components of asymptomatic and symptomatic banana plants infected by TR4.
CONTRIBUTOR : NOORADIBAH BINTI ISMAIL (11/05/2023 19:35 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 4706 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 6 ;
KEYWORD : [internal transcribed spacer] , [ rhizosphere] , [ richness] , [ soil microbiome] , [ soil physicochemical properties] , [ TR4]
4.
IN DEPTH ANALYSIS OF THE SOX4 GENE LOCUS THAT CONSISTS OF SENSE AND NATURAL ANTISENSE TRANSCRIPTS
DESCRIPTION : SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1], [2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.
CONTRIBUTOR : LING KING HWA (11/05/2023 17:05 PM)
FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2563 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 2 ;
KEYWORD : [Endogenous siRNA Brain development Natural antisense transcripts]
5.
DRAFT GENOME SEQUENCE OF MYO-INOSITOL UTILIZING AEROMONAS DHAKENSIS 1P11S3 ISOLATED FROM STRIPED CATFISH (PANGASIANODON HYPOPTHALMUS) IN A LOCAL FISH FARM IN MALAYSIA
DESCRIPTION : A hypervirulent pathotype of A. hydrophila (vAh) is responsible for Motile Aeromonas Septicemia (MAS) and causes mass mortalities among farmed carp and catfish species in the USA and China. One unique phenotype for vAh among other A. hydrophila strains is the ability to utilize myo-inositol as a sole carbon source. While screening for Aeromonas isolates from diseased fish that can grow using myo-inositol as a sole carbon source, A. dhakensis 1P11S3 was isolated from the spleen of striped catfish (Pangasianodon hypopthalmus) displaying clinical MAS symptoms from a freshwater farm in Malaysia. Aeromonas dhakensis is also an important pathogen in aquaculture, and in this study, we report the draft genome sequence for A. dhakensis 1P11S3, that utilize myo-inositol as a sole carbon source.
CONTRIBUTOR : MOHD TERMIZI BIN YUSOF (11/05/2023 17:00 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2707 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 0 ; DOWNLOAD : 0 ;
KEYWORD : [Draft genome] , [ myo-inositol A.] , [ dhakensis] , [ Malaysia]
6.
RNA-SEQUENCING OF LYMPH, SPLEEN, AND THYMUS TRANSCRIPTOME OF PENINSULAR MALAYSIA M. FASCICULARIS
DESCRIPTION : The cynomolgus macaque (Macaca fascicularis) is an extensively utilised nonhuman primate model for biomedical research due to its biological, behavioural, and genetic similarities to humans. Genomic information of cynomolgus macaque is vital for research in various fields; however, there is presently a shortage of genomic information on the Malaysian cynomolgus macaque. This study aimed to sequence, assemble, annotate, and profile the Peninsular Malaysian cynomolgus macaque transcriptome derived from three tissues (lymph node, spleen, and thymus) using RNA sequencing (RNA-Seq) technology. A total of 174,208,078 paired end 70 base pair sequencing reads were obtained from the Illumina Hi-Seq 2500 sequencer. The overall mapping percentage of the sequencing reads to the M. fascicularis reference genome ranged from 53-63%. Categorisation of expressed genes to Gene Ontology (GO) and KEGG pathway categories revealed that GO terms with the highest number of associated expressed genes include Cellular process, Catalytic activity, and Cell part, while for pathway categorisation, the majority of expressed genes in lymph node, spleen, and thymus fall under the Global overview and maps pathway category, while 266, 221, and 138 genes from lymph node, spleen, and thymus were respectively enriched in the Immune system category. Enriched Immune system pathways include Platelet activation pathway, Antigen processing and presentation, B cell receptor signalling pathway, and Intestinal immune network for IgA production. Differential gene expression analysis among the three tissues revealed 574 differentially expressed genes (DEG) between lymph and spleen, 5402 DEGs between lymph and thymus, and 7008 DEGs between spleen and thymus. Venn diagram analysis of expressed genes revealed a total of 2,630, 253, and 279 tissue-specific genes respectively for lymph node, spleen, and thymus tissues. This is the first time the lymph node, spleen, and thymus transcriptome of the Peninsular Malaysian cynomolgus macaque have been sequenced via RNA-Seq. Novel transcriptomic data will further enrich the present M. fascicularis genomic database and provide future research potentials, including novel transcript discovery, comparative studies, and molecular markers development.
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 16:25 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2702 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Genomic database; Lymph node; Macaca fascicularis; Peninsular malaysian; RNA sequencing; Spleen; Thymus; Transcriptome]
7.
FLORAL MICROMORPHOLOGY AND TRANSCRIPTOME ANALYSES OF A FRAGRANT VANDACEOUS ORCHID, VANDA MIMI PALMER, FOR ITS FRAGRANCE PRODUCTION SITES
DESCRIPTION : Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the flowers. Results Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifically linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at different time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. Conclusions Knowledge of the VMP floral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and flower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the flower morphology to maximize the reproduction success of this plant.
CONTRIBUTOR : PARAMESWARI A/P NAMASIVAYAM (11/05/2023 16:16 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2380 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Fragrance] , [ Monoterpene synthase] , [ Morphology] , [ Orchid] , [ Stomata] , [ Trichome]
8.
GENE EXPRESSION ANALYSIS REVEALS THE CONCURRENT ACTIVATION OF PRO-APOPTOTIC AND ANTIOXIDANT-DEFENSIVE MECHANISMS IN FLAVOKAWAIN B AND FIH-TREATED CERVICAL CANCER HELA CELLS
DESCRIPTION : To examine the difference in gene expression of HeLa cells after 48 hours of treatment with 7 µg/mL FKB and 15 µg/mL FIH after 48 hours. 3 sets of sample were prepared; the untreated HeLa cells, 7 µg/mL FKB-treated HeLa cells after 48 hours, 15 µg/mL of FIH-treated HeLa cells after 48 hours
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 14:58 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2555 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [HeLa cells] , [ cervical cancer]
9.
REGULATED GENE EXPRESSION FOR BREAST CANCER CELLS TREATED WITH NLC-CITRAL FOR 48 HOURS
DESCRIPTION : To further develop our gene expression study, we have employed the microrarray expression to study the regulate gene expression on breast cancer cells after 48 hours of treatments
CONTRIBUTOR : NOORJAHAN BANU BINTI MOHAMMED ALITHEEN (11/05/2023 14:48 PM)
FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2434 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [microrarray] , [ breast cancer] , [ NLC-Citral]
10.
SEPTICEMIA OF SUBTERRANEAN TERMITES COPTOTERMES CURVIGNATHUS CAUSED BY DISTURBANCE OF BACTERIA ISOLATED FROM TERMITE GUT AND ITS FORAGING PATHWAYS
DESCRIPTION : Microbial pathogens continue to attract a great deal of attention to manage termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remains elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106, and 109 CFU/mL and introduced to the termites via oral-contact and physical contact treatment. The data shows that contact method acted slower and gave lower mortality, compared to oral-contact method. C. curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU/mL via oral-contact and contact method, respectively. S. marcescens was also defined as the bacteria with the highest ability to induce high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and, to evaluate environmental and ecological risks of the biocontrol.
CONTRIBUTOR : H'NG PAIK SAN (11/05/2023 12:55 PM)
FACULTY : INSTITUT PERHUTANAN TROPIKA DAN PRODUK HUTAN
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2361 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Coptotermes curvignathus] , [ Serratia marcescens] , [ termite]
11.
PROFILING OF SPATIO-TEMPORALLY REGULATED TRANSCRIPTS DURING CEREBRAL CORTICOGENESIS IN THE MOUSE
DESCRIPTION : The cerebral cortex plays an important role in cognitive function and specialized perception in mammals and its development requires highly specific spatio-temporal control of gene expression. The study identified stage- and region-specific markers throughout cerebral corticogenesis at various important stages of cerebral cortex development; embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4-6 months old. The study involved the analysis of 12 SAGE libraries, which were generated from the mouse cerebral cortex of E15.5 (n=3), E17.5 (n=2), P1.5 (n=1) and 4-6 month old (n=6). N denotes biological replicates.
CONTRIBUTOR : LING KING HWA (11/05/2023 12:42 PM)
FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 2267 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [cerebral cortex] , [ spatio-temporal]
12.
EXPRESSION DATA FROM POSTNATAL MOUSE BRAIN REGIONS OF TS1CJE AND DISOMIC C57BL/6 MICE.
DESCRIPTION : The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. The mouse model develops various neuropathological features identified in DS individuals. We analysed the effect of partial triplication of the MMU16 segment on global gene expression in the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at 4 time-points; postnatal day (P)1, P15, P30 and P84.
CONTRIBUTOR : LING KING HWA (11/05/2023 12:26 PM)
FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS 
HAS VIEWS : 1081 ; FOLDER IN PROJECT : 3 ; FILE IN PROJECT : 3 ; DOWNLOAD : 0 ;
KEYWORD : [postnatal mouse brain] , [ mouse chromosome 16]

Organization (Faculty)

Fakulti Perubatan Dan Sains Kesihatan [25]
Fakulti Sains [18]
Fakulti Bioteknologi Dan Sains Biomolekul [6]
Fakulti Sains Komputer Dan Teknologi Maklumat [1]
Fakulti Bahasa Moden Dan Komunikasi [2]
Fakulti Kejuruteraan [2]
Institut Pertanian Tropika Dan Sekuriti Makanan [1]
Institut Gerontologi [2]
Institut Biosains [2]
Fakulti Sains Dan Teknologi Makanan [1]
Pusat Pembangunan Maklumat Dan Komunikasi [1]
Fakulti Ekonomi Dan Pengurusan [2]
Fakulti Perubatan Veterinar [4]
Fakulti Pertanian [8]
Fakulti Ekologi Manusia [2]
Institut Penyelidikan Produk Halal [1]
Institut Penyelidikan Matematik [1]
Fakulti Sains Pertanian Dan Makanan [2]
Institut Perhutanan Tropika Dan Produk Hutan [2]
Kampus Upm Bintulu [1]
Fakulti Perhutanan [6]

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Agricultural And Biological Sciences [13]
Biochemistry, Genetics And Molecular Biology [12]
Chemical Engineering [1]
Chemistry [11]
Computer Science [3]
Earth And Planetary Sciences [1]
Economics, Econometrics And Finance [1]
Energy [4]
Engineering [3]
Environmental Science [16]
Health Professions [1]
Immunology And Microbiology [4]
Materials Science [14]
Mathematics [1]
Medicine [29]
Pharmacology, Toxicology And Pharmaceutics [2]
Psychology [1]
Social Sciences [7]
Veterinary [5]