#RDRepo#

NO.	DATASET TITLE
1.	ASSOCIATION OF SOIL FUNGAL COMMUNITY COMPOSITION WITH INCIDENCE OF FUSARIUM WILT OF BANANA IN MALAYSIA DESCRIPTION : Banana (Musa spp.), an important food crop in many parts of the world, is threatened by a deadly wilt disease caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4). Increasing evidence indicates that plant actively recruits beneficial microbes in the rhizosphere to suppress soil-borne pathogens. Hence, studies on the composition and diversity of the root-associated microbial communities are important for banana health. Research on beneficial microbial communities has focused on bacteria, although fungi can also influence soil-borne disease. Here, high-throughput sequencing targeting the fungal internal transcribed spacer (ITS) was employed to systematically characterize the difference in the soil fungal community associated with Fusarium wilt (FW) of banana. The community structure of fungi in the healthy and TR4-infected rhizospheres was significantly different compared with that of bulk soil within the same farm. The rhizosphere soils of infected plants exhibited higher richness and diversity compared with healthy plants, with significant abundance of Fusarium genus at 14%. In the healthy rhizosphere soil, Penicillium spp. were more abundant at 7% and positively correlated with magnesium. This study produced a detailed description of fungal community structure in healthy and TR4-infected banana soils in Malaysia and identified candidate biomarker taxa that may be associated with FW disease promotion and suppression. The findings also expand the global inventory of fungal communities associated with the components of asymptomatic and symptomatic banana plants infected by TR4. CONTRIBUTOR : NOORADIBAH BINTI ISMAIL (11/05/2023 19:35 PM) FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 4709 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 6 ; KEYWORD : [internal transcribed spacer] , [ rhizosphere] , [ richness] , [ soil microbiome] , [ soil physicochemical properties] , [ TR4]
2.	SEPTICEMIA OF SUBTERRANEAN TERMITES COPTOTERMES CURVIGNATHUS CAUSED BY DISTURBANCE OF BACTERIA ISOLATED FROM TERMITE GUT AND ITS FORAGING PATHWAYS DESCRIPTION : Microbial pathogens continue to attract a great deal of attention to manage termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remains elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106, and 109 CFU/mL and introduced to the termites via oral-contact and physical contact treatment. The data shows that contact method acted slower and gave lower mortality, compared to oral-contact method. C. curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU/mL via oral-contact and contact method, respectively. S. marcescens was also defined as the bacteria with the highest ability to induce high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and, to evaluate environmental and ecological risks of the biocontrol. CONTRIBUTOR : H'NG PAIK SAN (11/05/2023 12:55 PM) FACULTY : INSTITUT PERHUTANAN TROPIKA DAN PRODUK HUTAN PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 2361 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Coptotermes curvignathus] , [ Serratia marcescens] , [ termite]
3.	GNPS - MITRAGYNA SPECIOSA ALKALOID FRACTION FROM MEOH CRUDE EXTRACT DESCRIPTION : The leave M. speciosa (Red vein) collected from Kedah, Malaysia were processed and extracted with MeOH. The MeOH crude extract was used to produced alkaloid fraction and this fraction was analysed on Q exactive plus LC-ms. [doi:10.25345/C51R0D] [dataset license: CC0 1.0 Universal (CC0 1.0)] CONTRIBUTOR : KHOZIRAH BINTI SHAARI (11/05/2023 12:07 PM) FACULTY : FAKULTI SAINS PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 2471 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Red vein] , [ MeOH] , [ M. speciosa ]
4.	DATASET OF TANDEM MASS SPECTRA FOR NON-HALAL PORK-BASED FOOD PRODUCTS DESCRIPTION : Shotgun proteomics is a high-throughput proteomic studies that commonly used to establish the peptide markers. Massive amounts of tandem mass spectra will be produced after an LC-MS/MS acquisition, as a consequence of thousands of proteins from several biological samples is digested by an enzyme. Manual interpretation for these massive mass spectra is unfeasible; instead, database search engine is used to match the spectra with sequence information contained in the database. Each search engine has a unique algorithm to provide a list of the best matching peptide sequences, as well as a score that related to the confidence level for an individual tandem mass spectrum. In this dataset, three proteomic database search engines namely Comet, X! Tandem, and ProteinProspector have been used against 45 types of pork-based food products with triplicate each for halal analysis. The dataset can be used to compare and discuss the ability of each database search engine, in term of detected spectra, matched peptide-spectrum, and inferred proteins. A false-discovery rate can also be compared and discussed from this dataset, in respect of database searching. A result of combined database search engine can be generated from this dataset to leverage the information contained in the database during interpretation of massive amounts of tandem mass spectra. CONTRIBUTOR : MOHD HAFIS YUSWAN BIN MOHD YUSOFF (11/05/2023 11:47 AM) FACULTY : INSTITUT PENYELIDIKAN PRODUK HALAL PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 2122 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 18 ; DOWNLOAD : 4 ; KEYWORD : [non halal] , [ pork based] , [ tandem mass]

1.

ASSOCIATION OF SOIL FUNGAL COMMUNITY COMPOSITION WITH INCIDENCE OF FUSARIUM WILT OF BANANA IN MALAYSIA
DESCRIPTION : Banana (Musa spp.), an important food crop in many parts of the world, is threatened by a deadly wilt disease caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4). Increasing evidence indicates that plant actively recruits beneficial microbes in the rhizosphere to suppress soil-borne pathogens. Hence, studies on the composition and diversity of the root-associated microbial communities are important for banana health. Research on beneficial microbial communities has focused on bacteria, although fungi can also influence soil-borne disease. Here, high-throughput sequencing targeting the fungal internal transcribed spacer (ITS) was employed to systematically characterize the difference in the soil fungal community associated with Fusarium wilt (FW) of banana. The community structure of fungi in the healthy and TR4-infected rhizospheres was significantly different compared with that of bulk soil within the same farm. The rhizosphere soils of infected plants exhibited higher richness and diversity compared with healthy plants, with significant abundance of Fusarium genus at 14%. In the healthy rhizosphere soil, Penicillium spp. were more abundant at 7% and positively correlated with magnesium. This study produced a detailed description of fungal community structure in healthy and TR4-infected banana soils in Malaysia and identified candidate biomarker taxa that may be associated with FW disease promotion and suppression. The findings also expand the global inventory of fungal communities associated with the components of asymptomatic and symptomatic banana plants infected by TR4.
CONTRIBUTOR : NOORADIBAH BINTI ISMAIL (11/05/2023 19:35 PM)

FACULTY : FAKULTI BIOTEKNOLOGI DAN SAINS BIOMOLEKUL
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 4709 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 6 ;
KEYWORD : [internal transcribed spacer] , [ rhizosphere] , [ richness] , [ soil microbiome] , [ soil physicochemical properties] , [ TR4]

2.

SEPTICEMIA OF SUBTERRANEAN TERMITES COPTOTERMES CURVIGNATHUS CAUSED BY DISTURBANCE OF BACTERIA ISOLATED FROM TERMITE GUT AND ITS FORAGING PATHWAYS
DESCRIPTION : Microbial pathogens continue to attract a great deal of attention to manage termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remains elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106, and 109 CFU/mL and introduced to the termites via oral-contact and physical contact treatment. The data shows that contact method acted slower and gave lower mortality, compared to oral-contact method. C. curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU/mL via oral-contact and contact method, respectively. S. marcescens was also defined as the bacteria with the highest ability to induce high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and, to evaluate environmental and ecological risks of the biocontrol.
CONTRIBUTOR : H'NG PAIK SAN (11/05/2023 12:55 PM)

FACULTY : INSTITUT PERHUTANAN TROPIKA DAN PRODUK HUTAN
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 2361 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Coptotermes curvignathus] , [ Serratia marcescens] , [ termite]

3.

GNPS - MITRAGYNA SPECIOSA ALKALOID FRACTION FROM MEOH CRUDE EXTRACT
DESCRIPTION : The leave M. speciosa (Red vein) collected from Kedah, Malaysia were processed and extracted with MeOH. The MeOH crude extract was used to produced alkaloid fraction and this fraction was analysed on Q exactive plus LC-ms. [doi:10.25345/C51R0D] [dataset license: CC0 1.0 Universal (CC0 1.0)]
CONTRIBUTOR : KHOZIRAH BINTI SHAARI (11/05/2023 12:07 PM)

FACULTY : FAKULTI SAINS
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 2471 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Red vein] , [ MeOH] , [ M. speciosa ]

4.

DATASET OF TANDEM MASS SPECTRA FOR NON-HALAL PORK-BASED FOOD PRODUCTS
DESCRIPTION : Shotgun proteomics is a high-throughput proteomic studies that commonly used to establish the peptide markers. Massive amounts of tandem mass spectra will be produced after an LC-MS/MS acquisition, as a consequence of thousands of proteins from several biological samples is digested by an enzyme. Manual interpretation for these massive mass spectra is unfeasible; instead, database search engine is used to match the spectra with sequence information contained in the database. Each search engine has a unique algorithm to provide a list of the best matching peptide sequences, as well as a score that related to the confidence level for an individual tandem mass spectrum. In this dataset, three proteomic database search engines namely Comet, X! Tandem, and ProteinProspector have been used against 45 types of pork-based food products with triplicate each for halal analysis. The dataset can be used to compare and discuss the ability of each database search engine, in term of detected spectra, matched peptide-spectrum, and inferred proteins. A false-discovery rate can also be compared and discussed from this dataset, in respect of database searching. A result of combined database search engine can be generated from this dataset to leverage the information contained in the database during interpretation of massive amounts of tandem mass spectra.
CONTRIBUTOR : MOHD HAFIS YUSWAN BIN MOHD YUSOFF (11/05/2023 11:47 AM)

FACULTY : INSTITUT PENYELIDIKAN PRODUK HALAL
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 2122 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 18 ; DOWNLOAD : 4 ;
KEYWORD : [non halal] , [ pork based] , [ tandem mass]

Fakulti Perubatan Dan Sains Kesihatan	[25]
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DATASETS IMMUNOLOGY AND MICROBIOLOGY

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DATASETS IMMUNOLOGY AND MICROBIOLOGY

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