#RDRepo#

NO.	DATASET TITLE
1.	MOLECULAR CHARACTERIZATION AND PATHOGENICITY OF NOVEL MALAYSIAN CHICKEN ASTROVIRUS ISOLATES DESCRIPTION : Chicken astrovirus (CAstV) has for over a decade been associated with runting stunting syndrome, severe kidney disease and visceral gout, and white chick syndrome. However, knowledge of the molecular characteristics and pathogenicity of the virus in day-old specific pathogen-free (SPF) chicks is scarce. This study focused on the characterization of near-complete genome of three Malaysian CAstV isolates following virus propagation in SPF embryonated chicken eggs and pathogenicity in day-old SPF chicks. The three isolates demonstrated unique features including a point mutation in their intergenic regions and an additional stem-loop II-like motif (s2m) in ORF-2. Pairwise sequence comparison and phylogenetic analysis of the ORF-2 amino acid sequence of the three isolates revealed an identity share of 86–91% with group B CAstVs while forming a new subgroup in addition to the known four subgroups (Bi, Bii, Biii and Biv) that exhibit high identity of between 95% and 100% within the subgroups. In the pathogenicity study, birds in the infected and exposed sentinel groups exhibited lethargy and diarrhoea 3 days post-inoculation (dpi) that declined by 6 dpi, and 20% growth retardation by 9 dpi. Mild lymphocytic aggregates in the duodenum, tubular degeneration and interstitial nephritis were observed in the intestines and kidneys, respectively, in both groups. In addition, the mean virus copy numbers of the cloacal swabs were log10 13.23 at 3 dpi and log10 9.04 at 6 dpi for the infected and exposed sentinels, respectively. The study suggests that the Malaysian isolates should be assigned to a new subgroup, Bv within group B CAstV. CONTRIBUTOR : ABDUL RAHMAN BIN OMAR (12/05/2023 11:01 AM) FACULTY : FAKULTI PERUBATAN VETERINAR PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 1670 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Chicken astrovirus] , [ group B CAstV] , [ genome sequencing] , [ molecular characterization] , [ pathogenicity] , [ SPF chicks]
2.	DEVELOPMENT AND MOLECULAR CHARACTERIZATION OF DOXORUBICIN-RESISTANT CANINE MAMMARY GLAND TUMOUR CELLS DESCRIPTION : Canine mammary gland tumour (CMT) commonly affects the female dog. The objective of this study was to develop a doxorubicin-resistant CMT cell line and determine its in vitro and in vivo characteristics, including mRNA and microRNA (miRNA) expression profiles. Doxorubicin-resistant CMT-Star cells were developed from CMT-Stylo cells. The cells were characterized, including tumorigenicity in NOD/SCID mouse models. MiRNA and mRNA expression of the two cell lines were profiled and clustered. ATP binding cassette subfamily B member 1 (ABCB1) and subfamily G member 2 (ABCG2) expressions were significantly increased in the CMT-Star cell line. CMT-Star cells also had altered expression of 785 genes and 14 miRNAs. Downregulating plasminogen (PLG) and plasminogen activator urokinase (PLAU) while upregulating transforming growth factor beta receptor 3 (TGFBR3), epidermal growth factor receptor 1 (EGFR1) and ABCB1 rendered CMT-Star cells less proliferative, less invasive and more resistant to chemotherapeutic drugs. The upregulated miRNAs in CMT-Star cells include miRNA-191, -29a, -107, -99b, -874, -93 and -210, while the downregulated miRNAs include miRNAs-106a, -92a, -92b, -155 and -15b. TGFβR, EGF receptor 1 and Wnt signalling are enriched in doxorubicin-resistant CMT-Star cells and could be potential therapeutic targets in dogs with doxorubicin-resistant CMT. CONTRIBUTOR : GAYATHRI THEVI A/P SELVARAJAH (11/05/2023 16:02 PM) FACULTY : FAKULTI PERUBATAN VETERINAR PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 1161 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Canine mammary cancer;drug resistance; geneexpression; doxorubicin;microarray; microRNA; cellline]
3.	MULTIPLE GENE TARGETING SIRNAS FOR DOWN REGULATION OF IMMEDIATE EARLY-2 (IE2) AND DNA POLYMERASE GENES MEDIATED INHIBITION OF NOVEL RAT CYTOMEGALOVIRUS (STRAIN ALL-03) DESCRIPTION : Cytomegalovirus (CMV) is an opportunistic pathogen that causes severe complications in congenitally infected newborns and non-immunocompetent individuals. Developing an effective vaccine is a major public health priority and current drugs are fronting resistance and side effects on recipients. In the present study, with the aim of exploring new strategies to counteract CMV replication, several anti-CMV siRNAs targeting IE2 and DNA polymerase gene regions were characterized and used as in combinations for antiviral therapy. Methods The rat embryo fibroblast (REF) cells were transfected with multi siRNA before infecting with CMV strain ALL-03. Viral growth inhibition was measured by tissue culture infectious dose (TCID50), cytopathic effect (CPE) and droplet digital PCR (ddPCR) while IE2 and DNA polymerase gene knockdown was determined by real-time PCR. Ganciclovir was deployed as a control to benchmark the efficacy of antiviral activities of respective individual siRNAs. Results There was no significant cytotoxicity encountered for all the combinations of siRNAs on REF cells analyzed by MTT colorimetric assay (P > 0.05). Cytopathic effects (CPE) in cells infected by RCMV ALL-03 had developed significantly less and at much slower rate compared to control group. The expression of targeted genes was downregulated successfully resulted in significant reduction (P < 0.05) of viral mRNA and DNA copies (dpb + dpc: 79%, 68%; dpb + ie2b: 68%, 60%; dpb + dpc + ie2b: 48%, 42%). Flow cytometry analysis showed a greater percentage of viable and early apoptosis of combined siRNAs-treated cells compared to control group. Notably, the siRNAs targeting gene regions were sequenced and mutations were not encountered, thereby avoiding the formation of mutant with potential resistant viruses. Conclusions In conclusion. The study demonstrated a tremendous promise of innovative approach with the deployment of combined siRNAs targeting at several genes simultaneously with the aim to control CMV replication in host cells. CONTRIBUTOR : MOHD AZMI BIN MOHD LILA (11/05/2023 15:43 PM) FACULTY : FAKULTI PERUBATAN VETERINAR PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 1852 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ; KEYWORD : [Cytomegalovirus] , [ Combinations] , [ Small interfering RNA] , [ Replication] , [ Gene expression]
4.	PROFILING OF SPATIO-TEMPORALLY REGULATED TRANSCRIPTS DURING CEREBRAL CORTICOGENESIS IN THE MOUSE DESCRIPTION : The cerebral cortex plays an important role in cognitive function and specialized perception in mammals and its development requires highly specific spatio-temporal control of gene expression. The study identified stage- and region-specific markers throughout cerebral corticogenesis at various important stages of cerebral cortex development; embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4-6 months old. The study involved the analysis of 12 SAGE libraries, which were generated from the mouse cerebral cortex of E15.5 (n=3), E17.5 (n=2), P1.5 (n=1) and 4-6 month old (n=6). N denotes biological replicates. CONTRIBUTOR : LING KING HWA (11/05/2023 12:42 PM) FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 2265 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ; KEYWORD : [cerebral cortex] , [ spatio-temporal]
5.	EXPRESSION DATA FROM POSTNATAL MOUSE BRAIN REGIONS OF TS1CJE AND DISOMIC C57BL/6 MICE. DESCRIPTION : The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. The mouse model develops various neuropathological features identified in DS individuals. We analysed the effect of partial triplication of the MMU16 segment on global gene expression in the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at 4 time-points; postnatal day (P)1, P15, P30 and P84. CONTRIBUTOR : LING KING HWA (11/05/2023 12:26 PM) FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN PUBLICATION : 0 ; FILE IN : HDFS HAS VIEWS : 1081 ; FOLDER IN PROJECT : 3 ; FILE IN PROJECT : 3 ; DOWNLOAD : 0 ; KEYWORD : [postnatal mouse brain] , [ mouse chromosome 16]

1.

MOLECULAR CHARACTERIZATION AND PATHOGENICITY OF NOVEL MALAYSIAN CHICKEN ASTROVIRUS ISOLATES
DESCRIPTION : Chicken astrovirus (CAstV) has for over a decade been associated with runting stunting syndrome, severe kidney disease and visceral gout, and white chick syndrome. However, knowledge of the molecular characteristics and pathogenicity of the virus in day-old specific pathogen-free (SPF) chicks is scarce. This study focused on the characterization of near-complete genome of three Malaysian CAstV isolates following virus propagation in SPF embryonated chicken eggs and pathogenicity in day-old SPF chicks. The three isolates demonstrated unique features including a point mutation in their intergenic regions and an additional stem-loop II-like motif (s2m) in ORF-2. Pairwise sequence comparison and phylogenetic analysis of the ORF-2 amino acid sequence of the three isolates revealed an identity share of 86–91% with group B CAstVs while forming a new subgroup in addition to the known four subgroups (Bi, Bii, Biii and Biv) that exhibit high identity of between 95% and 100% within the subgroups. In the pathogenicity study, birds in the infected and exposed sentinel groups exhibited lethargy and diarrhoea 3 days post-inoculation (dpi) that declined by 6 dpi, and 20% growth retardation by 9 dpi. Mild lymphocytic aggregates in the duodenum, tubular degeneration and interstitial nephritis were observed in the intestines and kidneys, respectively, in both groups. In addition, the mean virus copy numbers of the cloacal swabs were log10 13.23 at 3 dpi and log10 9.04 at 6 dpi for the infected and exposed sentinels, respectively. The study suggests that the Malaysian isolates should be assigned to a new subgroup, Bv within group B CAstV.
CONTRIBUTOR : ABDUL RAHMAN BIN OMAR (12/05/2023 11:01 AM)

FACULTY : FAKULTI PERUBATAN VETERINAR
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 1670 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Chicken astrovirus] , [ group B CAstV] , [ genome sequencing] , [ molecular characterization] , [ pathogenicity] , [ SPF chicks]

2.

DEVELOPMENT AND MOLECULAR CHARACTERIZATION OF DOXORUBICIN-RESISTANT CANINE MAMMARY GLAND TUMOUR CELLS
DESCRIPTION : Canine mammary gland tumour (CMT) commonly affects the female dog. The objective of this study was to develop a doxorubicin-resistant CMT cell line and determine its in vitro and in vivo characteristics, including mRNA and microRNA (miRNA) expression profiles. Doxorubicin-resistant CMT-Star cells were developed from CMT-Stylo cells. The cells were characterized, including tumorigenicity in NOD/SCID mouse models. MiRNA and mRNA expression of the two cell lines were profiled and clustered. ATP binding cassette subfamily B member 1 (ABCB1) and subfamily G member 2 (ABCG2) expressions were significantly increased in the CMT-Star cell line. CMT-Star cells also had altered expression of 785 genes and 14 miRNAs. Downregulating plasminogen (PLG) and plasminogen activator urokinase (PLAU) while upregulating transforming growth factor beta receptor 3 (TGFBR3), epidermal growth factor receptor 1 (EGFR1) and ABCB1 rendered CMT-Star cells less proliferative, less invasive and more resistant to chemotherapeutic drugs. The upregulated miRNAs in CMT-Star cells include miRNA-191, -29a, -107, -99b, -874, -93 and -210, while the downregulated miRNAs include miRNAs-106a, -92a, -92b, -155 and -15b. TGFβR, EGF receptor 1 and Wnt signalling are enriched in doxorubicin-resistant CMT-Star cells and could be potential therapeutic targets in dogs with doxorubicin-resistant CMT.
CONTRIBUTOR : GAYATHRI THEVI A/P SELVARAJAH (11/05/2023 16:02 PM)

FACULTY : FAKULTI PERUBATAN VETERINAR
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 1161 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Canine mammary cancer;drug resistance; geneexpression; doxorubicin;microarray; microRNA; cellline]

3.

MULTIPLE GENE TARGETING SIRNAS FOR DOWN REGULATION OF IMMEDIATE EARLY-2 (IE2) AND DNA POLYMERASE GENES MEDIATED INHIBITION OF NOVEL RAT CYTOMEGALOVIRUS (STRAIN ALL-03)
DESCRIPTION : Cytomegalovirus (CMV) is an opportunistic pathogen that causes severe complications in congenitally infected newborns and non-immunocompetent individuals. Developing an effective vaccine is a major public health priority and current drugs are fronting resistance and side effects on recipients. In the present study, with the aim of exploring new strategies to counteract CMV replication, several anti-CMV siRNAs targeting IE2 and DNA polymerase gene regions were characterized and used as in combinations for antiviral therapy. Methods The rat embryo fibroblast (REF) cells were transfected with multi siRNA before infecting with CMV strain ALL-03. Viral growth inhibition was measured by tissue culture infectious dose (TCID50), cytopathic effect (CPE) and droplet digital PCR (ddPCR) while IE2 and DNA polymerase gene knockdown was determined by real-time PCR. Ganciclovir was deployed as a control to benchmark the efficacy of antiviral activities of respective individual siRNAs. Results There was no significant cytotoxicity encountered for all the combinations of siRNAs on REF cells analyzed by MTT colorimetric assay (P > 0.05). Cytopathic effects (CPE) in cells infected by RCMV ALL-03 had developed significantly less and at much slower rate compared to control group. The expression of targeted genes was downregulated successfully resulted in significant reduction (P < 0.05) of viral mRNA and DNA copies (dpb + dpc: 79%, 68%; dpb + ie2b: 68%, 60%; dpb + dpc + ie2b: 48%, 42%). Flow cytometry analysis showed a greater percentage of viable and early apoptosis of combined siRNAs-treated cells compared to control group. Notably, the siRNAs targeting gene regions were sequenced and mutations were not encountered, thereby avoiding the formation of mutant with potential resistant viruses. Conclusions In conclusion. The study demonstrated a tremendous promise of innovative approach with the deployment of combined siRNAs targeting at several genes simultaneously with the aim to control CMV replication in host cells.
CONTRIBUTOR : MOHD AZMI BIN MOHD LILA (11/05/2023 15:43 PM)

FACULTY : FAKULTI PERUBATAN VETERINAR
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 1852 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 1 ; DOWNLOAD : 0 ;
KEYWORD : [Cytomegalovirus] , [ Combinations] , [ Small interfering RNA] , [ Replication] , [ Gene expression]

4.

PROFILING OF SPATIO-TEMPORALLY REGULATED TRANSCRIPTS DURING CEREBRAL CORTICOGENESIS IN THE MOUSE
DESCRIPTION : The cerebral cortex plays an important role in cognitive function and specialized perception in mammals and its development requires highly specific spatio-temporal control of gene expression. The study identified stage- and region-specific markers throughout cerebral corticogenesis at various important stages of cerebral cortex development; embryonic day (E) 15.5, E17.5, postnatal day (P) 1.5 and 4-6 months old. The study involved the analysis of 12 SAGE libraries, which were generated from the mouse cerebral cortex of E15.5 (n=3), E17.5 (n=2), P1.5 (n=1) and 4-6 month old (n=6). N denotes biological replicates.
CONTRIBUTOR : LING KING HWA (11/05/2023 12:42 PM)

FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 2265 ; FOLDER IN PROJECT : 1 ; FILE IN PROJECT : 4 ; DOWNLOAD : 0 ;
KEYWORD : [cerebral cortex] , [ spatio-temporal]

5.

EXPRESSION DATA FROM POSTNATAL MOUSE BRAIN REGIONS OF TS1CJE AND DISOMIC C57BL/6 MICE.
DESCRIPTION : The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. The mouse model develops various neuropathological features identified in DS individuals. We analysed the effect of partial triplication of the MMU16 segment on global gene expression in the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at 4 time-points; postnatal day (P)1, P15, P30 and P84.
CONTRIBUTOR : LING KING HWA (11/05/2023 12:26 PM)

FACULTY : FAKULTI PERUBATAN DAN SAINS KESIHATAN
PUBLICATION : 0 ; FILE IN : HDFS
HAS VIEWS : 1081 ; FOLDER IN PROJECT : 3 ; FILE IN PROJECT : 3 ; DOWNLOAD : 0 ;
KEYWORD : [postnatal mouse brain] , [ mouse chromosome 16]

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